Journal: Thoracic Cancer

Article Title: Harmonization across programmed death ligand 1 ( PD‐L1) assays for lung cancer by immunohistochemistry using noncontact alternating current electric field mixing

doi: 10.1111/1759-7714.13893

Figure Lengend Snippet: Comparison of the percentage of tumor cell staining for each programmed death ligand 1 (PD‐L1) assay (the 22C3, 28–8, SP263, and SP142 assays), by case, with/without alternating current (AC) mixing. (a) Conventional immunohistochemistry (IHC) ( ) 22C3, ( ) 28‐8, ( ) SP263, ( ) SP142. (b) AC mixing IHC ( ) 22C3, ( ) 28‐8 AC, ( ) SP263 AC, ( ) SP142 AC. The standard IHC is the 22C3 phamDx IHC on Autostainer Link 48 platform in both figures. Best fit colored curves enable comparison of score ranges between the four assays. When assessing PD‐L1 TPS in the cases of 22C3 PD‐L1 >1%‐positive patients using AC mixing, the nonlinear best fit curve calculated using the sigmoidal 4PL method showed one curve for all four PD‐L1 antibodies (extra sum‐of squares F test, p = 0.2552)

Article Snippet: Best fit curves data were fitted in GraphPad using the Sigmoidal 4PL option.

Techniques: Comparison, Staining, Immunohistochemistry

Journal: bioRxiv

Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

doi: 10.1101/2023.07.14.549076

Figure Lengend Snippet: A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Article Snippet: The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

Techniques: Neutralization, Infection, Incubation, Luciferase, Inhibition

Journal: Clinical & Translational Immunology

Article Title: Assessment of antibodies in the upper and lower human respiratory tract at steady state and after respiratory viral infection

doi: 10.1002/cti2.1460

Figure Lengend Snippet: Detection of antibodies in BAL samples and nasopharyngeal swabs. (a) ELISA curves from plasma, NP swab and BAL for total Ig (heavy and light chain), IgG, IgA and IgM. Measured optical density at 490 nm and a fitted sigmoidal 4 parameter logistic curves are shown. Each curve represents a different subject. The dotted horizontal line represents the cut‐off value for endpoint titre calculations (3× background signal). (b) Interpolated concentrations of IgG, IgA and IgM for paired plasma, NP swab and BAL samples. (c) Ratios of IgG and IgA concentrations in paired samples. Samples from control subjects were used for this figure ( n = 8). Statistical significance was determined by a Friedman's test with Dunn's correction for multiple comparisons.

Article Snippet: Concentrations were interpolated from a standard curve using a sigmoid 4PL curve in Graphpad Prism v9.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Control